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Mouse Integrin alpha 5/CD49e Alexa Fluor® 750-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1754S

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 235112

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha5/CD49e
Phe45-Asn999 (Gln68Glu)
Accession # P11688

Specificity

Detects mouse Integrin alpha5/CD49e in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse Integrin alpha 5/CD49e Alexa Fluor® 750-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: B16-F1 mouse melanoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Integrin alpha 5/CD49e

Integrin alpha5 subunit, also called CD49e, associates with the Integrin beta1 subunit (CD29) to form the VLA-5 complex. It is expressed on thymocytes, mast cells, activated T lymphocytes, and some mouse cell lines. It functions as a receptor for fibronectin (1‑4).

References

  1. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
  2. Kinashi, T. and T.A. Springer (1994) Blood Cells 20:25.
  3. Halvorson, M.J. et al. (1995) J. Immunol. 55:4567.
  4. Uhlenkott, E.C. et al. (1996) Clin. Exp. Metastasis 14:125.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Entrez Gene IDs

3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)

Gene Symbol

ITGA5

UniProt

Additional Integrin alpha 5/CD49e Products

Product Documents for Mouse Integrin alpha 5/CD49e Alexa Fluor® 750-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Integrin alpha 5/CD49e Alexa Fluor® 750-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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