Mouse Integrin beta1/CD29 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Rat
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 265917
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta1/CD29
Gln21-Asp728
Accession # P09055
Gln21-Asp728
Accession # P09055
Specificity
Detects mouse Integrin beta1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta1, recombinant mouse (rm) Integrin beta2, rmIntegrin beta3, rmIntegrin beta4, or rmIntegrin beta6 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Integrin beta1/CD29 Antibody
Detection of Integrin beta1/CD29 in B16‑F1 Mouse Cell Line by Flow Cytometry.
B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB2405, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Integrin beta1/CD29 in L‑929 Mouse Cell Line.
Integrin beta1/CD29 was detected in immersion fixed L-929 mouse fibroblast cell line using Rat Anti-Mouse Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB2405) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse Integrin beta1/CD29 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: B16-F10 mouse melanoma cell line
Sample: B16-F10 mouse melanoma cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed L-929 mouse fibroblast cell line
Sample: Immersion fixed L-929 mouse fibroblast cell line
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse spinal cord
Sample: Perfusion fixed frozen sections of mouse spinal cord
Western Blot
1 µg/mL
Sample: Recombinant Mouse Integrin beta1/CD29 under non-reducing conditions only
Sample: Recombinant Mouse Integrin beta1/CD29 under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 2.3 using MAB2405 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
Alternate Names
CD29, ITGB1
Gene Symbol
ITGB1
UniProt
Additional Integrin beta 1/CD29 Products
Product Documents for Mouse Integrin beta1/CD29 Antibody
Product Specific Notices for Mouse Integrin beta1/CD29 Antibody
For research use only
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