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Mouse Integrin  beta2/CD18 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2618

R&D Systems, part of Bio-Techne
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MAB2618
MAB2618-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

ELISA Development, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 313903

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta2/CD18
Gln24-Asn702
Accession # P11835

Specificity

Detects mouse Integrin beta2/CD18 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Integrin beta1, beta4, beta6, beta7, recombinant human (rh) Integrin beta2 or beta3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Integrin  beta2/CD18 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Integrin  beta2/CD18 under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 2/CD18

Integrin beta2 is a transmembrane protein expressed by all leukocytes. It occurs in heterodimeric complexes with Integrin subunits alphaD, alphaL (complex also known as LFA‑1), alphaM (Mac-1), or alphaX (CR-4). These complexes are important in adhesion and leukocyte trafficking.

Alternate Names

CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta

Entrez Gene IDs

3689 (Human); 16414 (Mouse); 309684 (Rat); 101925124 (Cynomolgus Monkey)

Gene Symbol

ITGB2

UniProt

Additional Integrin beta 2/CD18 Products

Product Documents for Mouse Integrin  beta2/CD18 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Integrin  beta2/CD18 Antibody

For research use only

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