Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha2b and Integrin beta3 linked heterodimer
Leu32-Arg988 (Integrin alpha2b) & Glu26-Asp717 (Integrin beta3)
Accession # NP_034705 (Integrin alpha2b) & O54890 (Integrin beta3)

Specificity

Detects mouse Integrin beta3/CD61 in direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for Mouse Integrin beta3/CD61 Antibody

Detection of Integrin beta3/CD61 in P815 Mouse Cell Line by Flow Cytometry.

P815 mouse mastocytoma cell line was stained with Rat Anti-Mouse Integrin beta3/CD61 Monoclonal Antibody (Catalog # MAB41182, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Applications for Mouse Integrin beta3/CD61 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/mL
Sample: P815 mouse mastocytoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 3/CD61

Integrin beta 3 (ITGB3) is also known as platelet glycoprotein IIIa (GPIIIa) and CD61. Integrins are integral cell-surface proteins known to participate in both cell adhesion and cell-surface mediated signaling. They are composed of an alpha chain and a beta chain. ITGB3 is an integrin beta chain that forms a heterodimer with alpha-IIb or alpha-V chains. It is involved in platelet aggregation, and serves as an anchor for fibrinogen, allowing platelets to form aggregates or clots. A functional absense of ITGB3 leads to Glanzmann's thrombasthenia, a condition where platelets are activated, but fail to form an aggregate. Alternatively spliced transcripts encoding different proteins have been described, in human.

Alternate Names

CD61, GP3A, GPIIIA, INGRB3, ITGB3

Entrez Gene IDs

3690 (Human); 16416 (Mouse); 29302 (Rat); 102117791 (Cynomolgus Monkey)

Gene Symbol

ITGB3

UniProt

NP_034705 (Integrin alpha2b) & O54890 (Integrin beta3)

Additional Integrin beta 3/CD61 Products

Product Documents for Mouse Integrin beta3/CD61 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Integrin beta3/CD61 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Integrin beta3/CD61 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB41182

Key Product Details

Species Reactivity

Validated:

Cited:

Applications