Skip to main content

Mouse Integrin  beta6 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2389

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AF2389-SP
Arrives in 7 - 9 Business Days
25 ug / $159.00
AF2389
 In Stock - Arrives in
1 - 2 Business Days
100 ug / $479.00

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry, Western Blot

Cited:

Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta6
Gly22-Asn706
Accession # Q9Z0T9

Specificity

Detects mouse Integrin beta6 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Integrin  beta6 Antibody

Detection of Human Integrin beta 6 by Western Blot

Detection of Human Integrin beta 6 by Western Blot

The effect of beta6 integrin-deficiency on inflammatory cytokine expression. (A) SiRNA knockdown of beta6 integrin in the GECs after a 48-h transfection relative to beta-actin detected by Western blotting. SiRNA that is not homologous to any human gene (C) was used as a control. (B) The effect of beta6 integrin knockdown on IL1B expression in biofilm-treated (60 µg protein/ml; 32 h) and non-treated GECs determined by RT-qPCR. Mean ± SEM is presented, n = 3. (C) The effect of TGF-beta 1 signaling inhibitor SB431542 (5 µM) on ITGB6 and IL1B expression in biofilm-treated (60 µg protein/ml; 32 h) and non-treated GECs. Mean ± SEM, n = 3. (D) GECs were treated with TGF-beta 1 (2 ng/ml), heated biofilm extract (60 µg protein/ml) or a combination of both for 32 h and analyzed for ITGB6 expression by RT-qPCR. Mean ± SEM, n = 3. (E) GECs were treated with TGF-beta 1 (2 ng/ml) for 0–120 min and analyzed for Smad2 phosphorylation (activation) relative to total Smad2 by Westerm blotting. (F and G) GECs were treated with TGF-beta 1 (2 ng/ml), heated biofilm extract (60 µg protein/ml) or a combination of both for 30 min and analyzed for Smad2 phosphorylation relative to total Smad2 by Westerm blotting. The ratio of phosphorylated Smad2 to total Smad2 was determined from triplicated experiments. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28667248), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Integrin  beta6 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Integrin  beta6

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 6

Integrin beta6 is a type I transmembrane Integrin beta subunit that heterodimerizes with alphaV subunits in the epithelial restricted integrin alphaV/ beta6. The extracellular domain of mouse Integrin beta6 contains a collagen binding von-Willebrand factor A domain and multiple truncated EGF-like motifs. Mouse Integrin beta6 extracellular domain shares 96% and 91% amino acid sequence identity with the corresponding regions of the rat and human protein, respectively.

Alternate Names

ITGB6

Entrez Gene IDs

3694 (Human); 16420 (Mouse); 311061 (Rat); 102122296 (Cynomolgus Monkey)

Gene Symbol

ITGB6

UniProt

Additional Integrin beta 6 Products

Product Documents for Mouse Integrin  beta6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Integrin  beta6 Antibody

For research use only

Loading...
Loading...
Loading...
Loading...
×