Mouse Intelectin-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5358

R&D Systems, part of Bio-Techne
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AF5358
AF5358-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Intelectin-2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Intelectin-2
Ala20-Ser298
Accession # Q80ZA0

Specificity

Detects mouse Intelectin-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant mouse Intelectin-1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Intelectin-2 Antibody

Detection of Mouse Intelectin-2 antibody by Western Blot.

Detection of Mouse Intelectin-2 by Western Blot.

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Intelectin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5358) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Intelectin-2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse Intelectin-2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse small intestine tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Intelectin-2

Intelectin-2 (ITLN-2, ITLN-1b and HL-2) is a 37-41 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by airway mucous cells plus intestinal Paneth and goblet cells, and appears to participate in fungal microbe recognition. The mouse ITLN-2 precursor is 313 amino acids (aa) in length. It contains a 19 aa signal sequence, a 279 aa mature region (aa 20-298), and a GPI-linked prodomain (aa 299-313). The mature region contains one C-terminal type fibrinogen domain (aa 32-251). Over aa 20-298, mouse ITLN-2 shares 79% aa sequence identity with human ITLN-2, and 92% aa sequence identity with mouse ITLN-1.

Alternate Names

HL-2, Intelectin-b, Intelectin2, Itln1b, ITLN2, Itlnb

Entrez Gene IDs

142683 (Human); 493583 (Mouse)

Gene Symbol

ITLN2

UniProt

Q80ZA0

Additional Intelectin-2 Products

Product Documents for Mouse Intelectin-2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Intelectin-2 Antibody

For research use only

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