A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse IRF5 Monoclonal Antibody (Catalog # MAB8447, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005).View our protocol for Staining Intracellular Molecules.

Detection of IRF5 in A20 cells by Flow Cytometry

A20 cells were stained with Rat Anti-Mouse IRF5 Monoclonal Antibody (Catalog # MAB8447, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Mouse IRF5 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: A20 mouse B cell lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005); A20 mouse B cell lymphoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer kit (FC012)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF5

IRF5 is a member of the IRF family of transcription factors, a family characterized by a helix-turn-helix DNA binding domain enriched in tryptophan repeats. IRF family members show diverse cellular regulation of interferon-stimulated gene transcription, viral-mediated gene activation, apoptosis, differentiation, and cellular growth. IRF5, along with IRF7, are the key mediators of TLR signaling. IRF5 forms heterodimers with IRF3 both of which are necessary for interferon gene transcription. IRF5 knock out mice indicate that IRF5 is critical for induction of apoptosis.

Long Name

Interferon Regulatory Factor 5

Alternate Names

IBD14, interferon regulatory factor 5, IRF-5, SLEB10

Entrez Gene IDs

3663 (Human); 27056 (Mouse); 296953 (Rat)

Gene Symbol

IRF5

UniProt

P56477

Additional IRF5 Products

Product Documents for Mouse IRF5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse IRF5 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse IRF5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8447

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IRF5 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse IRF5 Antibody

Detection of IRF5 in A20 Mouse Cell Line by Flow Cytometry.