Mouse IRF5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8447
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 903430
Product Specifications
Immunogen
E. coli-derived recombinant mouse IRF5
Ala208-Phe288
Accession # P56477
Ala208-Phe288
Accession # P56477
Specificity
Detects mouse IRF5 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse IRF5 Antibody
Detection of IRF5 in A20 Mouse Cell Line by Flow Cytometry.
A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse IRF5 Monoclonal Antibody (Catalog # MAB8447, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005).View our protocol for Staining Intracellular Molecules.Detection of IRF5 in A20 cells by Flow Cytometry
A20 cells were stained with Rat Anti-Mouse IRF5 Monoclonal Antibody (Catalog # MAB8447, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Mouse IRF5 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: A20 mouse B cell lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005); A20 mouse B cell lymphoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer kit (FC012)
Sample: A20 mouse B cell lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005); A20 mouse B cell lymphoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer kit (FC012)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRF5
Long Name
Interferon Regulatory Factor 5
Alternate Names
IBD14, interferon regulatory factor 5, IRF-5, SLEB10
Gene Symbol
IRF5
UniProt
Additional IRF5 Products
Product Documents for Mouse IRF5 Antibody
Product Specific Notices for Mouse IRF5 Antibody
For research use only
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