Mouse Kallikrein 5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7236
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 880036
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Kallikrein 5
23-293
23-293
Specificity
Detects mouse Kallikrein 5 in ELISA.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Kallikrein 5 Antibody
Detection of Mouse Kallikrein 5 by Western Blot.
Western blot shows lysates of NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Kallikrein 5 Monoclonal Antibody (Catalog # MAB7236) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Kallikrein 5 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Kallikrein 5 in Hair Follicle of Mouse Skin.
Kallikrein 5 was detected in immersion fixed frozen sections of mouse skin using Rat Anti-Mouse Kallikrein 5 Monoclonal Antibody (Catalog # MAB7236) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to the inner sheath cuticle of hair follicles. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Kallikrein 5 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse skin
Sample: Immersion fixed frozen sections of mouse skin
Western Blot
2 µg/mL
Sample: NMuMG mouse mammary gland epithelial cell line
Sample: NMuMG mouse mammary gland epithelial cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 5
References
- Brattsand M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
- Yousef G.M. et al. (2003) Cancer Res. 63:3958.
- Kim H. et al. (2001) Br. J. Cancer 84:543.
Alternate Names
KLK-L2, KLK5, SCTE
Gene Symbol
KLK5
Additional Kallikrein 5 Products
Product Documents for Mouse Kallikrein 5 Antibody
Product Specific Notices for Mouse Kallikrein 5 Antibody
For research use only
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