Mouse Kell Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1454
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Kell
Phe50-Trp713
Accession # Q9EQF2
Phe50-Trp713
Accession # Q9EQF2
Specificity
Detects mouse Kell in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Kell Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Kell (Catalog # 1454-ZN)
Sample: Recombinant Mouse Kell (Catalog # 1454-ZN)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kell
Kell, a type II membrane glycoprotein, is linked through a single disulfide bond to XK, a putative membrane transporter. The two proteins constitute the Kell blood group antigens (1). Kell is a zinc endopeptidase of the neprilysin (NEP) family which also includes endothelin converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE and several NEP-like proteins (2). It has been shown to cleave big endothelin-3 (ET-3) at Trp21-Ile22, yielding ET-3, and to a much lesser extent, big ET-1 and big ET-2 at Trp21-Val22, yielding ET-1 and ET-2 (3). Several different molecular defects cause the Kell null phenotype, which has no obvious clinical outcome (4).
References
- Lee, S. et al. (2000) Transfus. Med. Rev. 14:93.
- Turner, A.J. et al. (2001) BioEssays. 23:261.
- Lee, S. et al. (1999) Blood 94:1440.
- Lee, S. et al. (2001) J. Biol. Chem. 276:27281.
Long Name
Zinc protease blood group antigen
Alternate Names
CD238, KEL
Gene Symbol
KEL
UniProt
Additional Kell Products
Product Documents for Mouse Kell Biotinylated Antibody
Product Specific Notices for Mouse Kell Biotinylated Antibody
For research use only
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