Mouse Ki67/MKI67 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7649
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Ki67/MKI67
Asn3053-Ser3177
Accession # NP_001074586
Asn3053-Ser3177
Accession # NP_001074586
Specificity
Detects mouse Ki67/MKI67 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human Ki67/MKI67 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Ki67/MKI67 Antibody
Ki67/MKI67 in NIH‑3T3 Mouse Cell Line.
Ki67/MKI67 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Sheep Anti-Mouse Ki67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7649) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse Ki67/MKI67 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line
Sample: Immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF7649 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ki67/MKI67
MKI67 (also Ki67 and TSG126) is a 350-370 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki67 was originally recognized as an antigen associated with the monoclonal Ki67 antibody raised against Hodgkin's lymphoma nuclear material. Ki67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Mouse MKI67 is 3177 amino acids (aa) in length. It contains one FHA domain (aa 8-101), followed by sixteen 120 aa repeats (aa 993-2872). There are two potential isoform variants. One isoform shows a 19 aa substitution for 1120-3177, while a second isoform contains a deletion of aa 1169-1409. Over aa 3053-3177, mouse Ki67 shares 46% and 74% aa sequence identity with the human and rat orthologs to Ki67, respectively.
Long Name
Antigen Identified by Monoclonal Antibody Ki67
Alternate Names
Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126
Gene Symbol
MKI67
UniProt
Additional Ki67/MKI67 Products
Product Documents for Mouse Ki67/MKI67 Antibody
Product Specific Notices for Mouse Ki67/MKI67 Antibody
For research use only
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