Detects mouse L‑Selectin/CD62L in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human L-Selectin, recombinant mouse (rm) E-Selectin, or rmP-Selectin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Applications for Mouse L-Selectin/CD62L Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse splenocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: L-Selectin/CD62L

L‑Selectin (Leukocyte Selectin; also known as CD62L, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL‑14 antigen, DREG, and lymph node homing receptor), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L‑Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is
90‑100 kDa. Human and mouse L‑Selectin share 76% amino acid sequence homology. L‑Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L‑Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium. This initial interaction involving ICAM-1 and VCAM-1 leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble L‑Selectin are present in the biological fluids of apparently normal individuals. Levels of L‑Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Alternate Names

CD62L, hLHRc, LAM1, LECAM1, Leu-8, LNHR, LSEL, Lyam-1, LYAM1, PLNHR, SELL, TQ1

Entrez Gene IDs

6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)

Gene Symbol

SELL

UniProt

P18337

Additional L-Selectin/CD62L Products

Product Documents for Mouse L-Selectin/CD62L Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse L-Selectin/CD62L Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse L-Selectin/CD62L Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5761

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all L-Selectin/CD62L Antibodies »

Product Summary for Mouse L-Selectin/CD62L Antibody

Immunogen

Specificity