Mouse Laminin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB25491
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # AL-3
Product Specifications
Immunogen
Purified fragment of chymotrypsin-digested mouse EHS tumor-derived Laminin-1
Specificity
Detects mouse Laminin‑1 in Western blots. This antibody reacts with mouse Laminin-1 beta chain (1).
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse Laminin-1 Antibody
Laminin‑1 in Mouse Embryo.
Laminin-1 was detected in immersion fixed frozen sections of embryonic mouse parietal endoderm (E10.5) using Rat Anti-Mouse Laminin-1 Monoclonal Antibody (Catalog # MAB25491) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse Laminin-1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of mouse Engelbreth-Holm-Swarm tumor, and immersion fixed frozen sections of embryonic mouse parietal endoderm (E10.5)
Sample: Perfusion fixed paraffin-embedded sections of mouse Engelbreth-Holm-Swarm tumor, and immersion fixed frozen sections of embryonic mouse parietal endoderm (E10.5)
Western Blot
Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349. This application was not tested by R&D Systems.
Reviewed Applications
Read 2 reviews rated 5 using MAB25491 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin-1
Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes, and survival (1, 2).
References
- Skubitz, A.P. et al. (1988) J Biol Chem. 263:4861
- Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349.
Alternate Names
Laminin-111, Laminin1
Additional Laminin-1 Products
Product Documents for Mouse Laminin-1 Antibody
Product Specific Notices for Mouse Laminin-1 Antibody
For research use only
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