Mouse LAMP-1/CD107a Lumenal Domain Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4320
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LAMP1/CD107a
Leu25-Asn370
Accession # P11438
Leu25-Asn370
Accession # P11438
Specificity
Detects mouse LAMP1/CD107a Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant human LAMP1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse LAMP-1/CD107a Lumenal Domain Antibody
Detection of Mouse LAMP‑1/CD107a by Western Blot.
Western blot shows lysates of mouse small intestine tissue and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse LAMP-1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAMP-1/CD107a at approximately 100-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of LAMP-1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
LAMP1/CD107a in RAW 264.7 Mouse Cell Line.
LAMP1/CD107a was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse LAMP-1/CD107a Lumenal Domain Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: RAW 264.7 mouse monocyte/macrophage cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Western Blot
0.2 µg/mL
Sample: Mouse small intestine tissue and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: Mouse small intestine tissue and NIH‑3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using AF4320 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAMP-1/CD107a
LAMP-1 (lysosome-associated membrane glycoprotein 1; also P2B and CD107a) is a 120 kDa member of the LAMP family of proteins. It is a highly glycosylated type I transmembrane protein whose N-terminus projects either extracellularly or into the lumen of lysosomes. LAMP-1 may protect lysosomal membranes from enzymatic attack or mediate fusion of phagosomes with lysosomes. Mature mouse LAMP1 is 382 amino acids (aa) in length. It contains a 346 aa lumenal domain (aa 25‑370) and a 12 aa cytoplasmic region. Over aa 25‑370, mouse LAMP1 shares 82% and 62% aa identity with rat and human LAMP1, respectively.
Long Name
Lysosome-associated Membrane Glycoprotein 1
Alternate Names
CD107a, LAMP1
Gene Symbol
LAMP1
UniProt
Additional LAMP-1/CD107a Products
Product Documents for Mouse LAMP-1/CD107a Lumenal Domain Antibody
Product Specific Notices for Mouse LAMP-1/CD107a Lumenal Domain Antibody
For research use only
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