Mouse LIF Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB449

R&D Systems, part of Bio-Techne
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MAB449
MAB449-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry, Inhibition, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 139124

View all LIF Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse LIF
Pro25-Phe203

Specificity

Detects mouse LIF in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant mouse (rm) IL-6, rmIL-11, recombinant human (rh) LIF, rmCT-1, rhCLC or recombinant rat CNTF is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse LIF Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse LIF
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIF

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Entrez Gene IDs

3976 (Human); 16878 (Mouse); 403449 (Canine)

Gene Symbol

LIF

Additional LIF Products

Product Documents for Mouse LIF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse LIF Antibody

For research use only

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