Mouse Lipocalin-13 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7974

R&D Systems, part of Bio-Techne
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AF7974
AF7974-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Lipocalin-13 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Lipocalin-13
Ala19-Asp176
Accession # Q8K1H9

Specificity

Detects mouse Lipocalin-13 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Lipocalin-13 Antibody

Detection of Mouse Lipocalin-13 antibody by Western Blot.

Detection of Mouse Lipocalin-13 by Western Blot.

Western blot shows lysates of mouse liver tissue, mouse skeletal muscle tissue, mouse epididymis tissue, and mouse mammary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Lipocalin-13 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF7974) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lipocalin-13 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Lipocalin-13 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Mouse liver tissue, mouse skeletal muscle tissue, mouse epididymis tissue, and mouse mammary gland tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-13

LCN-13 (Lipocalin 13; also Odorant-binding protein 2a) is a secreted, 26-28 kDa member of the LCN family, calycin superfamily of molecules. Its name is derived from Greek, and denotes the fact that lipocalins are known to be "fat-wrapping", or fat binding proteins that interact with small lipophilic molecules within a central cavity. LCN-13 is expressed by hepatocytes and smooth muscle cells. It is known to have antidiabetogenic effects, including the suppression of hepatic glucose production, the uptake of circulating glucose by adipocytes, and the induction of fatty acid oxidation by liver, possibly by stimulating CPT1 alpha production. Mouse LCN‑13 is synthesized as a 176 amino acid (aa) precursor that contains a 19 aa signal sequence and a 157 aa mature region. Although no splice forms have been reported for LCN-13 to date, a smaller, presumably proteolytically processed 23-26 kDa isoform has been noted on SDS-PAGE. Mature mouse LCN-13 shares 69% and 39% aa sequence identity with rat LCN-13 and human odorant-binding protein 2b, respectively.

Alternate Names

LCN13, Lipocalin13, Obp2a

Entrez Gene IDs

227627 (Mouse)

Gene Symbol

OBP2A

UniProt

Q8K1H9 (Mouse)

Additional Lipocalin-13 Products

Product Documents for Mouse Lipocalin-13 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Lipocalin-13 Antibody

For research use only

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