Mouse Ly-6G (Gr-1) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10371
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
Flow Cytometry, Immunohistochemistry, In vivo assay, Neutralization, Neutrophil Depletion
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 1A8
Product Specifications
Immunogen
Ly-6G transfected EL-4J cell line
Accession # P35461
Accession # P35461
Specificity
Detects mouse Ly-6G in Flow Cytometry.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Ly-6G (Gr-1) Antibody
Detection of Ly-6G (GR-1) in Mouse Bone Marrow Cells by Flow Cytometry
Mouse bone marrow cells were stained with either (A) Rat Anti-Mouse Ly-6g (GR-1) Monoclonal Antibody (Catalog # MAB10371) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A).View our protocol for Staining Membrane-associated Proteins.Applications for Mouse Ly-6G (Gr-1) Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse bone marrow cells
Sample: Mouse bone marrow cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ly-6G (Gr-1)
References
- Spangrude, G.J. et al. (1988) Science 241:58.
- Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
Long Name
A Myeloid Differentiation Antigen
Alternate Names
Gr-1
Entrez Gene IDs
546644 (Mouse)
Gene Symbol
LY6G
UniProt
Additional Ly-6G (Gr-1) Products
Product Documents for Mouse Ly-6G (Gr-1) Antibody
Product Specific Notices for Mouse Ly-6G (Gr-1) Antibody
For research use only
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