Detects mouse Lymphotoxin betaR in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse (rm) 4‑1BB, rmBAFF R, rmCD27, rmCD30, rmCD40, recombinant human (rh) DR3, rhDR6, rmEDAR, rmFas, rmGITR, rmHVEM, rhLymphotoxin betaR, rmNGF R, rmOPG, rmOX40, rmRANK, rhRELT, rmTAJ, rmTNF RI, rmTNF RII, rhTRADD, rmTRAIL R2, rhTRAIL R3, rhTRAIL R4, or rmTWEAK R.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse Lymphotoxin betaR/TNFRSF3 Antibody

Detection of Lymphotoxin betaR/TNFRSF3 in NIH‑3T3 Mouse Cell Line by Flow Cytometry.

NIH-3T3 mouse embryonic fibroblast cell line was stained with Rat Anti-Mouse Lymphotoxin betaR/TNFRSF3 Monoclonal Antibody (Catalog # MAB10081, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Lymphotoxin betaR/TNFRSF3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: NIH‑3T3 mouse embryonic fibroblast cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse Lymphotoxin betaR/TNFRSF3
under non-reducing conditions only

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin betaR, now designated TNFRSF3, is a cell surface protein expressed in a variety of tissues. Lymphotoxin betaR transduces signals following binding of LIGHT or the heterotrimeric lymphotoxin LT alpha1/ beta2 or LT alpha2/ beta1. It plays a critical role in controlling cellular immune functions and lymphoid organogenesis.

Long Name

Lymphotoxin beta Receptor

Alternate Names

LTBR, LymphotoxinbR, TNF RIII, TNF Rrp, TNFRSF3

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Gene Symbol

LTBR

UniProt

P50284

Additional Lymphotoxin beta R/TNFRSF3 Products

Product Documents for Mouse Lymphotoxin betaR/TNFRSF3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Lymphotoxin betaR/TNFRSF3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Lymphotoxin betaR/TNFRSF3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10081

Key Product Details

Species Reactivity

Validated:

Cited:

Applications