Mouse LYVE-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2125
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
ELISA Detection, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Intravital Microscopy, Labeling, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 223322
Product Specifications
Immunogen
BaF/3 mouse pro-B cell line transfected with mouse LYVE-1
Ala24-Thr234
Accession # Q8BHC0
Ala24-Thr234
Accession # Q8BHC0
Specificity
Detects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD44 or recombinant human LYVE-1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse LYVE-1 Antibody
Detection of Mouse LYVE‑1 by Western Blot.
Western blot shows lysate of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LYVE-1 at approximately 65 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of LYVE‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
LYVE-1 in NS0 mouse myeloma cell line transfected with mouse LYVE-1.
LYVE-1 was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse LYVE-1 (positive control) and wild type NS0 mouse myeloma cell line (negative control) using Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse LYVE-1 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.02-0.1 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of biotinylated Hyaluronan to immobilized Recombinant Mouse LYVE-1 (Catalog # 2125-LY) coated at 5 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line
Sample: bEnd.3 mouse endothelioma cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed NS0 mouse myeloma cell line transfected with mouse LYVE-1
Sample: Immersion fixed NS0 mouse myeloma cell line transfected with mouse LYVE-1
Immunohistochemistry
Western Blot
2 µg/mL
Sample: bEnd.3 mouse endothelioma cell line under non-reducing conditions only
Sample: bEnd.3 mouse endothelioma cell line under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 5 using MAB2125 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LYVE-1
Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.
LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).
References
- Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
- Evered, D. and J. Whelan (1989) Ciba Found. Symp. 143:1.
- Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
- Banerji, S. et al. (1999) J. Cell Biol. 144:789.
- Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
- Carreira, C.M. et al. (2001) Cancer Research 61:8079.
- Jackson, D.J. et al. (2001) Trends Immunol. 22:317.
- Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
- Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
- Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
- Wiggle, J.T. and G. Oliver (1999) Cell 98:769.
Long Name
Lymphatic Vessel Endothelial Hyaluronan Receptor 1
Alternate Names
LYVE1, XLKD1
Gene Symbol
LYVE1
UniProt
Additional LYVE-1 Products
Product Documents for Mouse LYVE-1 Antibody
Product Specific Notices for Mouse LYVE-1 Antibody
For research use only
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