Mouse Meteorin-like/METRNL Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6679

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AF6679
AF6679-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Chromatin Immunoprecipitation (ChIP), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Meteorin-like/METRNL Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Meteorin-like/METRNL
Accession # Q8VE43

Specificity

Detects mouse Meteorin-like/METRNL in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Meteorin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Meteorin-like/METRNL Antibody

Meteorin-like/METRNL antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Meteorin‑like/METRNL in Mouse Embryo.

Meteorin-like/METRNL was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse Meteorin-like/METRNL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6679) at 0.6 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Meteorin-like/METRNL Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Meteorin-like/METRNL

Meteorin-like protein (METRNL) is a 311 amino acid (aa) protein that shows 45% homology with Meteorin (in mouse). Meteorin itself is a secreted 30 kDa protein that is expressed in glial and neuronal cells and their precursors during development and in the adult (1‑3). In the adult Meteorin is primarily expressed by astrocytes (3). Meteorin promotes neurite outgrowth, glial cell differentiation, and neuronal survival following excitotoxic injury (2‑5). It also inhibits sprouting angiogenesis and promotes blood vessel maturation (1). Mouse Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide and is selectively expressed in the otic vesicle during inner ear development (6, 7). Mature mouse Meteorin‑like protein shares 79% and 98% aa sequence identity with human and rat Meteorin‑like protein, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide. R&D Systems in‑house testing indicates that Meteorin‑like protein enhances the outgrowth of neurites from cultured mouse cortical neurons. The promoter region of the METRNL gene contains a recognition site for the Pax2, Pax5, and Pax8 transcription factors, and Pax2 binds specifically to this region (7). METRNL is the only gene found within the terminus of the human chromosome 17 q‑arm which can be deleted in the rare Ring 17 syndrome (8).

References

  1. Park, J.A. et al. (2008) Glia 56:247.
  2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104.
  3. Lee, H.S. et al. (2010) J. Cell Sci. 123:1959.
  4. Nishino, J. et al. (2004) EMBO J. 23:1998.
  5. Jorgensen, J.R. et al. (2011) Neurobiol. Dis. 41:160.
  6. Accession # Q8VE43.
  7. Ramialison, M. et al. (2008) Genome Biol. 9:R145.
  8. Surace, C. et al. (2009) Clin. Genet. 76:256.

Alternate Names

Meteorin like, METRNL

Entrez Gene IDs

284207 (Human); 210029 (Mouse); 316842 (Rat)

Gene Symbol

METRNL

UniProt

Q8VE43

Additional Meteorin-like/METRNL Products

Product Documents for Mouse Meteorin-like/METRNL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Meteorin-like/METRNL Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.