Mouse MICL/CLEC12A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2950
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 812303
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MICL/CLEC12A with an N-terminal 9-His tag
Thr67-Arg267
Accession # Q504P2
Thr67-Arg267
Accession # Q504P2
Specificity
Detects mouse MICL/CLEC12A in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse MICL/CLEC12A Antibody
Detection of MICL/CLEC12A in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with either (A) Rat Anti-Mouse MICL/CLEC12A Monoclonal Antibody (Catalog # MAB2950) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Goat Anti-Rat PE-conjugated secondary antibody (Catalog # F0105B) and Rat Anti-Mouse CD11c APC-conjugated Monoclonal Antibody (Catalog # FAB69501A). Cells were first gated on Rat Anti-Mouse CD8 alpha Alexa Fluor® 488-conjugated Monoclonal Antibody-positive cells (Catalog # FAB116G, not shown) . View our protocol for Staining Membrane-associated Proteins.Applications for Mouse MICL/CLEC12A Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: CD8+ CD11c+ Mouse Splenocytes
Sample: CD8+ CD11c+ Mouse Splenocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MICL/CLEC12A
MICL, also known as killer cell lectin-like receptor 1 (KLR1), is an inhibitory C-type lectin-like receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). It is a75 kDa type II membrane protein that is expressed on neutrophils, NK cells, monocytes and dendritic cells. The amino acid sequence of mouse MICL extracellular domain is 53%, 52% and 58% identical to that of human, bovine and canine MICL, respectively.
Long Name
Myeloid Inhibitory C-type Lectin-like Receptor
Alternate Names
CD371, CLEC12A, CLL-1, DCAL-2
Gene Symbol
CLEC12A
UniProt
Additional MICL/CLEC12A Products
Product Documents for Mouse MICL/CLEC12A Antibody
Product Specific Notices for Mouse MICL/CLEC12A Antibody
For research use only
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