Mouse MICL/CLEC12A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2950
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MICL/CLEC12A
Thr67-Arg267
Accession # NP_808354
Thr67-Arg267
Accession # NP_808354
Specificity
Detects recombinant mouse MICL/CLEC12A in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than1% cross‑reactivity with recombinant human MICL is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse MICL/CLEC12A Antibody
Detection of MICL/CLEC12A in M1 Mouse Cell Line by Flow Cytometry.
M1 mouse myeloid leukemia cell line was stained with Goat Anti-Mouse MICL/CLEC12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2950, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
MICL/CLEC12A in Mouse Splenocytes.
MICL/CLEC12A was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse MICL/CLEC12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2950) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse MICL/CLEC12A Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: M1 mouse myeloid leukemia cell line
Sample: M1 mouse myeloid leukemia cell line
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse MICL/CLEC12A
Sample: Recombinant Mouse MICL/CLEC12A
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MICL/CLEC12A
MICL, also known as killer cell lectin-like receptor 1 (KLR1), is an inhibitory C-type lectin-like receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). It is a75 kDa type II membrane protein that is expressed on neutrophils, NK cells, monocytes and dendritic cells. The amino acid sequence of mouse MICL extracellular domain is 53%, 52% and 58% identical to that of human, bovine and canine MICL, respectively.
Long Name
Myeloid Inhibitory C-type Lectin-like Receptor
Alternate Names
CD371, CLEC12A, CLL-1, DCAL-2
Gene Symbol
CLEC12A
UniProt
Additional MICL/CLEC12A Products
Product Documents for Mouse MICL/CLEC12A Antibody
Product Specific Notices for Mouse MICL/CLEC12A Antibody
For research use only
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