Mouse Nectin-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3116

R&D Systems, part of Bio-Techne
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AF3116
AF3116-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Hamster

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Nectin-4 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Nectin-4
Tyr28-Ile349
Accession # Q8R007

Specificity

Detects mouse Nectin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Nectin-4 is observed and less than 5% cross-reactivity with recombinant mouse Nectin-1, -2, and -3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Nectin-4 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Nectin-4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-4

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of mouse Nectin-4 shares 90% and 95% amino acid sequence homology with the corresponding regions of human and rat Nectin-4, respectively.

Long Name

Poliovirus Receptor Related 4

Alternate Names

LNIR, Nectin4, PRR4, PVRL4

Entrez Gene IDs

81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)

Gene Symbol

NECTIN4

UniProt

Q8R007

Additional Nectin-4 Products

Product Documents for Mouse Nectin-4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Nectin-4 Antibody

For research use only

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