Mouse Nogo-B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6596
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Nogo-B
Met1-Val183
Accession # NP_918941
Met1-Val183
Accession # NP_918941
Specificity
Detects mouse Nogo-B in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Nogo-B and recombinant rat (rr) Nogo-A (aa 2-172) is observed and less than 1% cross-reactivity with rhNogo-A, rrNogo-A (aa 544-725), and rrNogo-A (aa 1026-1091) is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Nogo-B Antibody
Nogo‑B in Mouse Embryonic Retina.
Nogo-B was detected in immersion fixed frozen sections of mouse embryonic retina (E13.5) using Sheep Anti-Mouse Nogo-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6596) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to retina. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Mouse Nogo‑B by Western Blot.
Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Nogo-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6596) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nogo-B at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse Nogo-B Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion-fixed frozen sections of mouse embryonic retina
Sample: Immersion-fixed frozen sections of mouse embryonic retina
Western Blot
1 µg/mL
Sample: Neuro‑2A mouse neuroblastoma cell line and C2C12 mouse myoblast cell line
Sample: Neuro‑2A mouse neuroblastoma cell line and C2C12 mouse myoblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nogo-B
Nogo-B (A No-Go for neurite outgrowth Isoform B; also reticulon-4 isoform 2 and RTN-xS) is a 49-51 kDa member of the reticulon protein family. It is widely expressed, being reported everywhere but liver. Nogo-B appears to be principally expressed in the ER, but does have a receptor (NgBR) on cell surfaces. Intracellularly, Nogo-B is known to interact with Bcl-xl and Bcl-2, and may play in role in both apoptosis and angiogenesis. Mouse NOGO-B/B1 is a two transmembrane, 356 amino acid protein. It contains an N-terminal cytoplasmic domain (aa 1-182), two transmembrane segments (aa 183-203 and 273-293), an intervening luminal region (aa 204-272) and a C-terminal cytoplasmic domain (aa 294-356). Nogo-B contains multiple phosphorylation sites and undergoes acetylation. Caspase-7 cleaves Nogo-B between Asp15Ser16, generating a 40-42 kDa fragment. Nogo-B exists in two forms; B1 represents aa 1-168 spliced to aa 975-1162 of Nogo-A, while B2 represents aa 1-187 spliced to aa 975-1162 of Nogo-A. Over aa 1-183, mouse Nogo-B/B1 shares 76% aa identity with human Nogo-B/B1.
Long Name
Reticulon 4B
Alternate Names
NogoB, RTN4B
Gene Symbol
RTN4
UniProt
Additional Nogo-B Products
Product Documents for Mouse Nogo-B Antibody
Product Specific Notices for Mouse Nogo-B Antibody
For research use only
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