Mouse Osteoactivin/GPNMB Antibody

Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody.

Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC), immobilized onto a microplate previously coated with Goat Anti-Mouse IgG Fc (Catalog # G-202-C), supports the adhesion of the SVEC4-10 mouse vascular endothelial cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Osteoactivin/ GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330). The ND₅₀ is typically 0.2-0.8 µg/mL.

Detection of Human Osteoactivin/GPNMB by Western Blot

GPNMB and galectin-3 levels are elevated in FTD-GRN brains. a, b GPNMB and galectin-3 levels (ng/mg protein) were measured in frontal lobe tissue lysates generated from cognitively normal controls (CTL; n = 27) and FTD-GRN patients (n = 25). Data analyzed using unpaired t-test. c Representative immunoblots for GPNMB and galectin-3 in frontal lobe lysates from cognitively normal controls (n = 8) and FTD-GRN (n = 8) patients. d GPNMB levels (ng/mL) in CSF samples form cognitively normal controls (n = 14), FTD-GRN (n = 9), FTD-C9orf72 (n = 12) and FTD-MAPT (n = 12) samples quantified by ELISA. Data analyzed using one-way ANOVA. e, f GPNMB immunostaining was performed on frontal lobe tissue sections from cognitively normal controls (n = 5) (e) and FTD-GRN (n = 5) (f) patients. g, h Immunostaining for p-TDP 43 was stained on adjacent sections from identical samples in e, f as marker of FTLD pathology. i GPNMB staining intensity in human brain sections (e, f) were measured and presented as fold change. Representative immunofluorescence staining for cell markers (green) (j, n, r), GPNMB (red) (k, o, s), DAPI (blue) (i, p, t) in paraffin sections of brains from FTD-GRN cases. Iba-1, GFAP, NeuN used for markers of human microglia, astrocytes, and neurons respectively. GPNMB and Iba-1 signals overlap (arrow) (m) whereas, no overlapping signal was observed in co-staining with GFAP or NeuN (q, u). Scale bars were labeled in the images. Data analyzed by unpaired t-test. Scale bars (20 µm) labeled in images and quantitative data are shown as mean ± SEM, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33028409), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Osteoactivin/GPNMB by Immunohistochemistry

GPNMB and galectin-3 co-localize with Iba-1 positive microglia cells in Grn−/− mouse brain. a Representative immunofluorescent co-staining for different cell markers (Iba-1, microglia; GFAP, astrocytes; NeuN, neurons) are shown in green, GPNMB (red), and nuclei (DAPI; blue) in brains of 19-month-old Grn−/− mice. b Representative immunofluorescent co-staining for different cell markers (Iba-1, microglia; GFAP, astrocytes; NeuN, neurons) are shown in green, galectin-3 (red), and nuclei (DAPI; blue) in brains of 19-month-old Grn−/− mice. Scale bars (20 µm) labeled in images Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33028409), licensed under a CC-BY license. Not internally tested by R&D Systems.