Mouse P-Cadherin Antibody

Detection of P-Cadherin in Human Liver.

P-Cadherin was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Mouse P-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Mouse P‑Cadherin by Simple Western^TM.

Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. A specific band was detected for P-Cadherin at approximately 115 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse P-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF761) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse P-Cadherin by Western Blot

BMPR1a regulated P-cadherin expression via p63 and Slug. (A) Immunohistochemistry staining for P-cadherin in control (n = 4 mice) and cKO (n = 3 mice) mammary glands at pregnancy day 14.5. Scale bar, 50 μm. (B) Western blotting for P-cadherin in mammary epithelial cells isolated from control and cKO mice at pregnancy day 14.5. beta-Tubulin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Tubulin. n = 3 mice. (C) qRT-PCR analysis of Cdh3 in FACS-sorted control and cKO myoepithelial cells at pregnancy day 14.5. n = 4 biological replicates. (D) P-cadherin (red) and K14 (green) double immunofluorescence staining in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. n = 3 biological replicates. Scale bar, 25 μm. (E) Western blotting for P-cadherin in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. beta-Tubulin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Tubulin. n = 3 biological replicates. (F) Scatter plot showing the correlation between BMPR1A and CDH3 expression in mammary glands from TCGA and GTEx data. Pearson’s coefficient test was performed to assess statistical significance. (G) Western blotting for P-cadherin in HC11 cells treated with p63 siRNA (sip63)/Slug siRNA (siSlug) and scramble RNA (NC) at 48 h. beta-Actin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Actin. n = 3 biological replicates. (H) Immunofluorescence for P-cadherin (green) in HC11 cells treated with p63 siRNA (sip63)/Slug siRNA (siSlug) and scramble RNA (NC) at 48 h. n = 3 biological replicates. Scale bar, 25 μm. (I) Western blotting for K14, P-cadherin, p63 and Slug in HC11 cells treated with scramble RNA, p63 siRNA, Slug siRNA, or p63 siRNA and Slug siRNA at 48 h. beta-Actin was used as a loading control. Statistical analysis the expression of K14/ beta-Actin, P-cadherin/ beta-Actin, p63/ beta-Actin and Slug/ beta-Actin. n = 3 biological replicates. Data were presented as means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34336839), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse P-Cadherin by Western Blot

BMPR1a regulated P-cadherin expression via p63 and Slug. (A) Immunohistochemistry staining for P-cadherin in control (n = 4 mice) and cKO (n = 3 mice) mammary glands at pregnancy day 14.5. Scale bar, 50 μm. (B) Western blotting for P-cadherin in mammary epithelial cells isolated from control and cKO mice at pregnancy day 14.5. beta-Tubulin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Tubulin. n = 3 mice. (C) qRT-PCR analysis of Cdh3 in FACS-sorted control and cKO myoepithelial cells at pregnancy day 14.5. n = 4 biological replicates. (D) P-cadherin (red) and K14 (green) double immunofluorescence staining in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. n = 3 biological replicates. Scale bar, 25 μm. (E) Western blotting for P-cadherin in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. beta-Tubulin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Tubulin. n = 3 biological replicates. (F) Scatter plot showing the correlation between BMPR1A and CDH3 expression in mammary glands from TCGA and GTEx data. Pearson’s coefficient test was performed to assess statistical significance. (G) Western blotting for P-cadherin in HC11 cells treated with p63 siRNA (sip63)/Slug siRNA (siSlug) and scramble RNA (NC) at 48 h. beta-Actin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Actin. n = 3 biological replicates. (H) Immunofluorescence for P-cadherin (green) in HC11 cells treated with p63 siRNA (sip63)/Slug siRNA (siSlug) and scramble RNA (NC) at 48 h. n = 3 biological replicates. Scale bar, 25 μm. (I) Western blotting for K14, P-cadherin, p63 and Slug in HC11 cells treated with scramble RNA, p63 siRNA, Slug siRNA, or p63 siRNA and Slug siRNA at 48 h. beta-Actin was used as a loading control. Statistical analysis the expression of K14/ beta-Actin, P-cadherin/ beta-Actin, p63/ beta-Actin and Slug/ beta-Actin. n = 3 biological replicates. Data were presented as means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34336839), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse P-Cadherin by Western Blot

BMPR1a regulated P-cadherin expression via p63 and Slug. (A) Immunohistochemistry staining for P-cadherin in control (n = 4 mice) and cKO (n = 3 mice) mammary glands at pregnancy day 14.5. Scale bar, 50 μm. (B) Western blotting for P-cadherin in mammary epithelial cells isolated from control and cKO mice at pregnancy day 14.5. beta-Tubulin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Tubulin. n = 3 mice. (C) qRT-PCR analysis of Cdh3 in FACS-sorted control and cKO myoepithelial cells at pregnancy day 14.5. n = 4 biological replicates. (D) P-cadherin (red) and K14 (green) double immunofluorescence staining in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. n = 3 biological replicates. Scale bar, 25 μm. (E) Western blotting for P-cadherin in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. beta-Tubulin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Tubulin. n = 3 biological replicates. (F) Scatter plot showing the correlation between BMPR1A and CDH3 expression in mammary glands from TCGA and GTEx data. Pearson’s coefficient test was performed to assess statistical significance. (G) Western blotting for P-cadherin in HC11 cells treated with p63 siRNA (sip63)/Slug siRNA (siSlug) and scramble RNA (NC) at 48 h. beta-Actin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Actin. n = 3 biological replicates. (H) Immunofluorescence for P-cadherin (green) in HC11 cells treated with p63 siRNA (sip63)/Slug siRNA (siSlug) and scramble RNA (NC) at 48 h. n = 3 biological replicates. Scale bar, 25 μm. (I) Western blotting for K14, P-cadherin, p63 and Slug in HC11 cells treated with scramble RNA, p63 siRNA, Slug siRNA, or p63 siRNA and Slug siRNA at 48 h. beta-Actin was used as a loading control. Statistical analysis the expression of K14/ beta-Actin, P-cadherin/ beta-Actin, p63/ beta-Actin and Slug/ beta-Actin. n = 3 biological replicates. Data were presented as means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34336839), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse P-Cadherin by Western Blot

BMPR1a regulated P-cadherin expression via p63 and Slug. (A) Immunohistochemistry staining for P-cadherin in control (n = 4 mice) and cKO (n = 3 mice) mammary glands at pregnancy day 14.5. Scale bar, 50 μm. (B) Western blotting for P-cadherin in mammary epithelial cells isolated from control and cKO mice at pregnancy day 14.5. beta-Tubulin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Tubulin. n = 3 mice. (C) qRT-PCR analysis of Cdh3 in FACS-sorted control and cKO myoepithelial cells at pregnancy day 14.5. n = 4 biological replicates. (D) P-cadherin (red) and K14 (green) double immunofluorescence staining in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. n = 3 biological replicates. Scale bar, 25 μm. (E) Western blotting for P-cadherin in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. beta-Tubulin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Tubulin. n = 3 biological replicates. (F) Scatter plot showing the correlation between BMPR1A and CDH3 expression in mammary glands from TCGA and GTEx data. Pearson’s coefficient test was performed to assess statistical significance. (G) Western blotting for P-cadherin in HC11 cells treated with p63 siRNA (sip63)/Slug siRNA (siSlug) and scramble RNA (NC) at 48 h. beta-Actin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Actin. n = 3 biological replicates. (H) Immunofluorescence for P-cadherin (green) in HC11 cells treated with p63 siRNA (sip63)/Slug siRNA (siSlug) and scramble RNA (NC) at 48 h. n = 3 biological replicates. Scale bar, 25 μm. (I) Western blotting for K14, P-cadherin, p63 and Slug in HC11 cells treated with scramble RNA, p63 siRNA, Slug siRNA, or p63 siRNA and Slug siRNA at 48 h. beta-Actin was used as a loading control. Statistical analysis the expression of K14/ beta-Actin, P-cadherin/ beta-Actin, p63/ beta-Actin and Slug/ beta-Actin. n = 3 biological replicates. Data were presented as means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34336839), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse P-Cadherin by Western Blot

BMPR1a regulated P-cadherin expression via p63 and Slug. (A) Immunohistochemistry staining for P-cadherin in control (n = 4 mice) and cKO (n = 3 mice) mammary glands at pregnancy day 14.5. Scale bar, 50 μm. (B) Western blotting for P-cadherin in mammary epithelial cells isolated from control and cKO mice at pregnancy day 14.5. beta-Tubulin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Tubulin. n = 3 mice. (C) qRT-PCR analysis of Cdh3 in FACS-sorted control and cKO myoepithelial cells at pregnancy day 14.5. n = 4 biological replicates. (D) P-cadherin (red) and K14 (green) double immunofluorescence staining in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. n = 3 biological replicates. Scale bar, 25 μm. (E) Western blotting for P-cadherin in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. beta-Tubulin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Tubulin. n = 3 biological replicates. (F) Scatter plot showing the correlation between BMPR1A and CDH3 expression in mammary glands from TCGA and GTEx data. Pearson’s coefficient test was performed to assess statistical significance. (G) Western blotting for P-cadherin in HC11 cells treated with p63 siRNA (sip63)/Slug siRNA (siSlug) and scramble RNA (NC) at 48 h. beta-Actin was used as a loading control. Statistical analysis the expression of P-cadherin/ beta-Actin. n = 3 biological replicates. (H) Immunofluorescence for P-cadherin (green) in HC11 cells treated with p63 siRNA (sip63)/Slug siRNA (siSlug) and scramble RNA (NC) at 48 h. n = 3 biological replicates. Scale bar, 25 μm. (I) Western blotting for K14, P-cadherin, p63 and Slug in HC11 cells treated with scramble RNA, p63 siRNA, Slug siRNA, or p63 siRNA and Slug siRNA at 48 h. beta-Actin was used as a loading control. Statistical analysis the expression of K14/ beta-Actin, P-cadherin/ beta-Actin, p63/ beta-Actin and Slug/ beta-Actin. n = 3 biological replicates. Data were presented as means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34336839), licensed under a CC-BY license. Not internally tested by R&D Systems.