Mouse PACAP/ADCYAP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6380
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse PACAP/ADCYAP1
Pro26-Leu175
Accession # O70176
Pro26-Leu175
Accession # O70176
Specificity
Detects mouse PACAP/ADCYAP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 12% cross‑reactivity with recombinant human PACAP is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse PACAP/ADCYAP1 Antibody
Detection of Mouse PACAP/ADCYAP1 by Western Blot.
Western blot shows lysates of mouse kidney tissue. PVDF Membrane was probed with 1 µg/mL of Human PACAP/ADCYAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PACAP/ADCYAP1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2Applications for Mouse PACAP/ADCYAP1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse kidney tissue
Sample: Mouse kidney tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PACAP/ADCYAP1
PACAP (Pituitary adenylate cyclase activating polypeptide) is a 17 kDa (predicted) member of the VIP/secretin/glucagon/GHRH superfamily of peptides. It is expressed by anterior pituitary gonadotrophs, neurons in the CNS and PNS, spermatids, adrenal chromaffin cells, plus macrophages and lymphocytes. PACAP has multiple effects. On mast cells, it induces histamine release; on lymphocytes, PACAP promotes Th2 development; in bone, PACAP inhibits osteoclastogenesis; and in the pituitary, PACAP facilitates the release of LH (in females), GH and alpha‑MSH. Mouse PACAP proprecursor is 151 amino acids (aa) in length (aa 25‑175). It undergoes proteolytic processing to generate two C‑terminal amidated peptides termed PACAP-38 (aa 131‑168) and PACAP-27 (aa 131‑157). In general, PACAP-38 represents the dominant form. However, the ratio between the -38 and -27 forms differs in a cell-specific manner. Over aa 25‑175, mouse PACAP shares 95% and 81% aa identity with rat and human PACAP, respectively.
Long Name
Pituitary Adenylate Cyclase-activating Polypeptide
Alternate Names
ADCYAP1
Gene Symbol
ADCYAP1
UniProt
Additional PACAP/ADCYAP1 Products
Product Documents for Mouse PACAP/ADCYAP1 Antibody
Product Specific Notices for Mouse PACAP/ADCYAP1 Antibody
For research use only
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