Mouse Patched 2/PTCH2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8096

R&D Systems, part of Bio-Techne
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MAB8096
MAB8096-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 865619

View all Patched 2/PTCH2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Patched 2/PTCH2
Glu79-Ser394
Accession # O35595

Specificity

Detects mouse Patched 2/PTCH2 in ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Patched 1 or recombinant human Patched 2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Patched 2/PTCH2 Antibody

Patched 2/PTCH2 antibody in Mouse Testis by Immunohistochemistry (IHC-Fr).

Patched 2/PTCH2 in Mouse Testis.

Patched 2/PTCH2 was detected in immersion fixed frozen sections of mouse testis using Rat Anti-Mouse Patched 2/PTCH2 Monoclonal Antibody (Catalog # MAB8096) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse Patched 2/PTCH2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse testis

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Patched 2/PTCH2

Patched 2 (Ptch2) is a 180-200 kDa 12 transmembrane member of the Patched family. It is less widely expressed than Patched 1, but is found at high levels in skin and spermatocytes. The 1182 amino acid (aa) mouse Patched 2 contains two terminal cytoplasmic domains between aa 1-57 and aa 1108-1182. Together with Smoothened (SMO), Patched 2 forms a membrane complex that serves as a receptor for Sonic Hedgehog (Shh). Over the first extracellular loop (aa 79-394), mouse Patched 2 shares 95% aa sequence identity with human Patched 2. Mouse Patched 2 has one potential 1117 aa isoform that lacks aa 207-271.

Alternate Names

ptc2, PTCH2

Entrez Gene IDs

8643 (Human); 19207 (Mouse)

Gene Symbol

PTCH2

UniProt

O35595

Additional Patched 2/PTCH2 Products

Product Documents for Mouse Patched 2/PTCH2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Patched 2/PTCH2 Antibody

For research use only

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