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Mouse Plasminogen Kringle 5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB742

R&D Systems, part of Bio-Techne
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MAB742
MAB742-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 120304

Product Specifications

Immunogen

E. coli-derived recombinant mouse Plasminogen Kringle 5
Cys481-Ala563
Accession # AAA50168

Specificity

Detects mouse Plasminogen Kringle 5 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human Plasminogen Kringle 5 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse Plasminogen Kringle 5 Antibody

Detection of Mouse Plasminogen Kringle 5 by Western Blot

Detection of Mouse Plasminogen Kringle 5 by Western Blot

Ferulic acid regulated the differential expression of angiogenic and antiangiogenic factors in MN mouse model.  At the end of the experiment, kidney samples of different groups were harvested and freshly isolated the glomeruli according to the Materials and Methods. Total protein lysate was extracted from isolated glomeruli and separated in 10% SDS page by electrophoresis, and further blotting by anti-VEGF (a), TSP-1 (b), and K5 (c) antibodies. The bar chart represented the semiquantification level of the expression density (target/actin) (Left Panel) (*P < 0.05; **P < 0.01). Immunofluorescence staining was also undertaken to exam the expression level and location of VEGF, TSP-1, and K5 in NC and N-T, original magnifications, 400x (Right Panel). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22844329), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Plasminogen Kringle 5 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Plasminogen Kringle 5

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plasminogen Kringle 5

Plasminogen is a coenzyme that has five triple disulfide-linked kringle domains. It is converted to plasmin by plasminogen activators. Internal fragments of plasminogen, including the naturally occurring angiostatin isoforms and Kringle 5, can also be generated by proteolytic cleavage. These internal fragments have been shown to be potent angiogenesis inhibitors (1).

References

  1. Cao, Y. et al. (1997) J. Biol. Chem. 272:22924.

Alternate Names

HAE4

Entrez Gene IDs

5340 (Human); 18815 (Mouse); 85253 (Rat)

Gene Symbol

PLG

UniProt

Additional Plasminogen Kringle 5 Products

Product Documents for Mouse Plasminogen Kringle 5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Plasminogen Kringle 5 Antibody

For research use only

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