Mouse PLET-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6917
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 700738
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PLET-1
Ala27-Leu216
Accession # Q8VEN2
Ala27-Leu216
Accession # Q8VEN2
Specificity
Detects mouse PLET-1 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse PLET-1 Antibody
Detection of PLET-1 in Mouse Thymocytes by Flow Cytometry.
Mouse thymocytes were stained with Rat Anti-Mouse PLET-1 Monoclonal Antibody (Catalog # MAB6917, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).PLET‑1 in Mouse Kidney.
PLET-1 was detected in immersion fixed frozen sections of adult mouse kidney using Rat Anti-Mouse PLET-1 Monoclonal Antibody (Catalog # MAB6917) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to medulla collecting ducts. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse PLET-1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse thymocytes
Sample: Mouse thymocytes
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of adult mouse kidney
Sample: Immersion fixed frozen sections of adult mouse kidney
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLET-1
Long Name
Placenta-expressed Transcript 1
Alternate Names
AgK114, PLET1
Entrez Gene IDs
76509 (Mouse)
Gene Symbol
PLET1
UniProt
Additional PLET-1 Products
Product Documents for Mouse PLET-1 Antibody
Product Specific Notices for Mouse PLET-1 Antibody
For research use only
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