Mouse PLTP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4918
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PLTP
Glu18-Ala493 (Asp32Glu)
Accession # P55065
Glu18-Ala493 (Asp32Glu)
Accession # P55065
Specificity
Detects mouse PLTP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human PLTP is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse PLTP Antibody
PLTP in Mouse Brain.
PLTP was detected in perfusion fixed frozen sections of mouse brain (medulla) using Sheep Anti-Mouse PLTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4918) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse PLTP Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (medulla)
Sample: Perfusion fixed frozen sections of mouse brain (medulla)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse PLTP (Catalog # 4918-PL)
Sample: Recombinant Mouse PLTP (Catalog # 4918-PL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLTP
PLTP (phospholipid transfer protein) is a 65-80 kDa, secreted glycoprotein member of the BPI/LBP family of proteins. It is expressed by multiple cell types, circulates bound to HDL, and mediates the transfer of phospholipids and cholesterol from apoB-containing lipoproteins to HDL. Mature mouse PLTP is 476 amino acids (aa) in length. It contains an N-terminal lipid transfer domain (aa 21-239), a C-terminal HDL-binding domain (aa 263-463), and one essential intrachain disulfide bond (Cys146‑Cys185). There are three potential PLTP splice variants. One shows a deletion of aa 148-493, a second shows a deletion of aa 111-162, and a third shows a deletion of aa 14-33 plus a two aa substitution for aa 184-493. Over aa 18-493, mouse PLTP shares 92% and 83% aa identity with rat and human PLTP, respectively.
Long Name
Phospholipid Transfer Protein
Alternate Names
HDLCQ9, Lipid transfer protein II, phospholipid transfer protein
Gene Symbol
PLTP
UniProt
Additional PLTP Products
Product Documents for Mouse PLTP Antibody
Product Specific Notices for Mouse PLTP Antibody
For research use only
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