Mouse PLZF Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8395

R&D Systems, part of Bio-Techne
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MAB8395
MAB8395-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 816416

View all PLZF Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse PLZF
Met1-Gln254
Accession # NP_001028496

Specificity

Detects mouse PLZF in direct ELISA and Western Blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse PLZF Antibody

Detection of Human and Mouse PLZF antibody by Western Blot.

Detection of Human and Mouse PLZF by Western Blot.

Western blot shows lysates of BaF3 mouse pro-B cell line and KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse PLZF Monoclonal Antibody (Catalog # MAB8395) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PLZF at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse PLZF Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: BaF3 mouse pro-B cell line and KG‑1 human acute myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLZF

Mouse PLZF, also known as Zinc finger and BTB domain containing protein 16 (ZBTB-16) PLZF and ZNF145, is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid (aa) protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc finger domain. PLZF forms dimers with RAR alpha and LAZ3 within its zinc finger region. Alternate splice forms exist that are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. PLZF is highly expressed in undifferentiated, multi-potential hematopoietic progenitor cells, with levels declining as cells commit to various lineages. Mouse PLZF shares 98% and 96% aa identity with rat and human PLZF, respectively.

Long Name

Promyelocytic Leukemia Zinc Finger

Alternate Names

ZBTB16, ZNF145

Entrez Gene IDs

7704 (Human); 235320 (Mouse)

Gene Symbol

ZBTB16

UniProt

NP_001028496

Additional PLZF Products

Product Documents for Mouse PLZF Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse PLZF Antibody

For research use only

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