Mouse splenocytes stimulated with Recombinant Mouse IL-12 (Catalog # 419-ML) and Recombinant Mouse IL-18 (Catalog # 9139-IL) for 3 days were stained with Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB22252P) and either (A) Rat Anti-Mouse PLZF Monoclonal Antibody (Catalog # MAB29441) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Mouse PLZF Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse splenocytes stimulated with Recombinant Mouse IL‑12 (Catalog # 419-ML) and Recombinant Mouse IL-18 (Catalog # 9139-IL) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLZF

Mouse PLZF, also known as Zinc finger and BTB domain containing protein 16 (ZBTB-16) PLZF and ZNF145, is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid (aa) protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc finger domain. PLZF forms dimers with RAR alpha and LAZ3 within its zinc finger region. Alternate splice forms exist that are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. PLZF is highly expressed in undifferentiated, multi-potential hematopoietic progenitor cells, with levels declining as cells commit to various lineages. Mouse PLZF shares 98% and 96% aa identity with rat and human PLZF, respectively.

Long Name

Promyelocytic Leukemia Zinc Finger

Alternate Names

ZBTB16, ZNF145

Entrez Gene IDs

7704 (Human); 235320 (Mouse)

Gene Symbol

ZBTB16

UniProt

NP_001028496

Additional PLZF Products

Product Documents for Mouse PLZF Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse PLZF Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse PLZF Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB29441

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PLZF Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse PLZF Antibody

Detection of PLZF in Mouse Splenocytes by Flow Cytometry.