Podocalyxin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using 10 µg/mL Rat Anti-Mouse Podocalyxin Monoclonal Antibody (Catalog # MAB1556) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Podocalyxin in Mouse Kidney Tissue.

Podocalyxin was detected in perfusion fixed frozen sections of mouse kidney tissue using Rat Anti-Mouse Podocalyxin Monoclonal Antibody (Catalog # MAB1556) at 1.7 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC005) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse Podocalyxin Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: D3 mouse embryonic stem cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed bEnd.3 mouse endothelioma cell line

Immunohistochemistry

1-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney tissue

Western Blot

1 µg/mL
Sample: Recombinant Mouse Podocalyxin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using MAB1556 in the following applications:

Immunohistochemistry-Frozen (2 Reviews)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References

Li, J. et al. (2001) DNA Seq. 12(5):407.
Hara, T. et al. (1999) Immunity 11(5):567.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Entrez Gene IDs

5420 (Human); 27205 (Mouse)

Gene Symbol

PODXL

UniProt

Q9R0M4

Additional Podocalyxin Products

Product Documents for Mouse Podocalyxin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Podocalyxin Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Podocalyxin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1556

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Podocalyxin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Podocalyxin Antibody

Podocalyxin in bEnd.3 Mouse Cell Line.