Mouse Podocalyxin Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1556

R&D Systems, part of Bio-Techne
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BAF1556
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AF1556
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Western Blot

Cited:

Cell Isolation, Cell Selection, Flow Cytometry, MACS

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Podocalyxin Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
His22-Arg402
Accession # Q9R0M4

Specificity

Detects mouse Podocalyxin in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Podocalyxin Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: D3 mouse embryonic stem cell line

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver and thymus

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Podocalyxin
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References

  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Entrez Gene IDs

5420 (Human); 27205 (Mouse)

Gene Symbol

PODXL

UniProt

Q9R0M4

Additional Podocalyxin Products

Product Documents for Mouse Podocalyxin Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Podocalyxin Biotinylated Antibody

For research use only

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