Mouse Pref-1/DLK1/FA1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8277
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Pref-1/DLK1/FA1
Ala24-Gln305
Accession # Q09163
Ala24-Gln305
Accession # Q09163
Specificity
Detects mouse Pref‑1/DLK1/FA1 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant human Pref-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Pref-1/DLK1/FA1 Antibody
Detection of Mouse Pref‑1/DLK1/FA1 by Western Blot.
Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Pref-1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pref-1/DLK1/FA1 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry.
3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Goat Anti-Mouse Pref-1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Pref‑1/DLK1/FA1 in Mouse Embryo.
Pref-1/DLK1/FA1 was detected in immersion fixed frozen sections of mouse embryonic lung using Goat Anti-Mouse Pref-1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Pref-1/DLK1/FA1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryonic lung
Sample: Immersion fixed frozen sections of mouse embryonic lung
Western Blot
1 µg/mL
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pref-1/DLK1/FA1
Pref-1 (Preadipocyte factor 1, Protein delta homolog 1, DLK1, FA1 and Fetal antigen 1) is a 45-60 kDa transmembrane glycoprotein that is highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary. Expression of Pref-1 is elevated in liver after birth but starts to decline around postnatal day 16. It contains 6 EGF-like domains and is involved in embryonic skeletal system development. Pref-1 inhibits preadipocyte proliferation by regulating their entry into G1/S-phase and represses preadipocyte differentiation. It is a master regulator of preadipocyte homeostasis and adipose tissue expansion. Pref-1 manipulation may, therefore, be utilized in obesity treatments. Mouse Pref-1 shares 81% aa identity with human Pref-1.
Long Name
Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1
Alternate Names
DLK-1, DLK1, FA1, pG2, Pref1, ZOG
Gene Symbol
DLK1
UniProt
Additional Pref-1/DLK1/FA1 Products
Product Documents for Mouse Pref-1/DLK1/FA1 Antibody
Product Specific Notices for Mouse Pref-1/DLK1/FA1 Antibody
For research use only
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