3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Rabbit Anti-Mouse Pref-1/DLK1/FA1 PE-conjugated Monoclonal Antibody (Catalog # FAB8634P, filled histogram) or isotype control antibody (Catalog # IC105P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Pref-1/DLK1/FA1 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pref-1/DLK1/FA1

Pref-1 (Preadipocyte factor 1), also known as Protein delta homolog 1, DLK1, FA1 and Fetal antigen 1, is a 45-60 kDa transmembrane glycoprotein that is highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary. Expression of Pref-1 is elevated in liver after birth but starts to decline around postnatal day 16. Mature mouse Pref-1 is a 362 amino acid (aa) type I transmembrane N- and O-linked glycoprotein. It contains a 282 aa extracellular region (aa 24-305), a 24 aa transmembrane segment (aa 306-329), and a 56 aa cytoplasmic domain (aa 330-385). It contains 6 EGF-like domains and is involved in embryonic skeletal system development. Pref-1 inhibits preadipocyte proliferation by regulating their entry into G1/S-phase and represses preadipocyte differentiation. It is a master regulator of preadipocyte homeostasis and adipose tissue expansion. Pref-1 manipulation may, therefore, be utilized in obesity treatments. Mouse Pref-1 shares 85% and 94% aa identity with human and rat Pref-1, respectively.

Long Name

Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1

Alternate Names

DLK-1, DLK1, FA1, pG2, Pref1, ZOG

Entrez Gene IDs

8788 (Human); 13386 (Mouse)

Gene Symbol

DLK1

UniProt

Q09163

Additional Pref-1/DLK1/FA1 Products

Product Documents for Mouse Pref-1/DLK1/FA1 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Pref-1/DLK1/FA1 PE-conjugated Antibody

For research use only

Citations
Reviews

Calculators
Spectra Viewer
Flow Cytometry

Mouse Pref-1/DLK1/FA1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8634P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Pref-1/DLK1/FA1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Pref-1/DLK1/FA1 PE-conjugated Antibody

Detection of Pref-1/DLK1/FA1 in 3T3-L1 Mouse Cell Line by Flow Cytometry.