Mouse Protocadherin-12 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7926

R&D Systems, part of Bio-Techne
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AF7926
AF7926-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Protocadherin-12 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Protocadherin-12
Leu18-Ala716
Accession # O55134

Specificity

Detects mouse Protocadherin-12 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human PCDH-18 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Protocadherin-12 Antibody

Detection of Mouse Protocadherin-12 antibody by Western Blot.

Detection of Mouse Protocadherin-12 by Western Blot.

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Protocadherin-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7926) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Protocadherin-12 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Protocadherin-12 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: bEnd.3 mouse endothelioma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-12

PCDH12 (Protocadherin 12; also VE‑Cadherin 2) is a 150‑160 kDa glycoprotein member of the PCDH family of molecules. It exhibits limited expression, being found on endothelial cells, renal mesangium, Sertoli cells, and glycogen‑positive trophoblast cells. PCDH is a transmembrane protein that forms homotypic aggregates in a calcium‑dependent manner. Although this would suggest a prominent role in cell-cell adhesion, it is unclear if this is the only activity for the molecule. In any case, it appears that PCDH12 does play an important role in placental development. Mature mouse PCDH12 is an 1163 amino acid (aa) type I transmembrane protein (aa 18‑1180). It possesses a 699 aa extracellular region (aa 18‑716) that contains six cadherin domains (aa 28‑711), plus a 21 aa transmembrane segment coupled to a lengthy 443 aa cytoplasmic domain (aa 738‑1180). Based on human, mouse PCDH12 will undergo proteolytic processing. MMP activity will first generate a 90 kDa circulating extracellular fragment and 60 kDa membrane‑bound fragment, followed by gamma‑secretase which has the potential to further act on the membrane‑bound fragment, generating a cytosolic 50 kDa fragment. There is one potential alternative start site at Met1060. Over aa 18‑716, mouse PCDH12 shares 95% and 82% aa sequence identity with rat and human PCDH12, respectively.

Alternate Names

PCDH12, Protocadherin12, VE-cad-2, VE-cadherin-2

Entrez Gene IDs

51294 (Human); 53601 (Mouse)

Gene Symbol

PCDH12

UniProt

O55134 (Mouse)

Additional Protocadherin-12 Products

Product Documents for Mouse Protocadherin-12 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Protocadherin-12 Antibody

For research use only

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