Mouse Protogenin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4919
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PRTG
Val24-Thr943
Accession # Q2EY15
Val24-Thr943
Accession # Q2EY15
Specificity
Detects mouse and human PRTG in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Protogenin Antibody
Protogenin in Embryonic Mouse Neural Tube.
Protogenin was detected in immersion fixed frozen sections of embryonic mouse neural tube (E9) using 10 µg/mL Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Protogenin in Mouse Neural Tube.
Protogenin was detected in immersion fixed frozen sections of mouse neural tube (E9.5) using Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Protogenin in Mouse Embryo.
Protogenin was detected in immersion fixed paraffin-embedded 13.5 d.p.c sections of Mouse Embryo using Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membranes in neuronal cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Mouse Protogenin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse neural tube (E9) and immersion fixed paraffin-embedded 13.5 d.p.c sections of Mouse Embryo
Sample: Immersion fixed frozen sections of embryonic mouse neural tube (E9) and immersion fixed paraffin-embedded 13.5 d.p.c sections of Mouse Embryo
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Protogenin
Sample: Recombinant Mouse Protogenin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protogenin
Protogenin (Early/proto in expression, neogenin-like; also Prtg) is a 130 kDa (predicted), type I transmembrane member of the DCC/Neogenin family of proteins. It is expressed in mouse presomitic mesoderm, paraxial mesoderm, and neural tube. Mature mouse protogenin is 1168 amino acids (aa) in length. It contains a 920 aa extracellular domain (ECD) (aa 24-943) plus a 224 aa cytoplasmic region. The ECD contains four consecutive Ig-like domains (aa 24-405), followed by five sequential fibronectin type III domains (aa 413-908). The ECD of mouse protogenin shares 98%, 87%, and 93% aa sequence identity with the ECD of rat, chick, and human protogenin, respectively.
Alternate Names
IGDCC5, PRTG, RPTG, Shen-Dan
Gene Symbol
PRTG
UniProt
Additional Protogenin Products
Product Documents for Mouse Protogenin Antibody
Product Specific Notices for Mouse Protogenin Antibody
For research use only
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