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Mouse R-Spondin 3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4120

R&D Systems, part of Bio-Techne
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MAB4120
MAB4120-SP

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 400404

Product Specifications

Immunogen

E. coli-derived recombinant mouse R‑Spondin 3
Ser21-Gly209 (Gln108Lys)
Accession # BAC36296

Specificity

Detects mouse R‑Spondin 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) RSPO-1, rmRSPO-4, recombinant human (rh) RSPO-2, or rhRSPO-3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse R-Spondin 3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse R-Spondin 3

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: R-Spondin 3

RSPO-3 (R-spondin 3; also Cristin-1), is a 256 aa secreted R-Spondin family member which binds LRP-6 and Fzd8 and regulates beta-catenin signaling. R-Spondins contain two adjacent cysteine-rich furin-like domains at their amino-termini, followed by a thrombospondin (TSP-1) motif and a variable length basic C-terminal region. Two RSPO-3 splice isoforms that differ in their C-termini have been reported. Mouse RSPO-3 shares 93% and 97% aa identity with human and rat RSPO-3, respectively, over the sequence used as an immunogen.

Long Name

Roof Plate-specific Spondin 3

Alternate Names

Cristin 1, PWTSR, RSPO3, RSpondin 3, THSD2

Entrez Gene IDs

84870 (Human); 72780 (Mouse); 102132009 (Cynomolgus Monkey)

Gene Symbol

RSPO3

UniProt

Additional R-Spondin 3 Products

Product Documents for Mouse R-Spondin 3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse R-Spondin 3 Antibody

For research use only

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