Mouse RANK/TNFRSF11A Biotinylated Antibody

Detection of RANK/TNFRSF11A by Flow Cytometry

RANK expression levels under adherent and non-adherent conditions.A. BMMs under the adherent condition were harvested (0 h) and subsequently cultured again under adherent and non-adherent conditions in the presence or absence of M-CSF (50 ng/ml), TGF-beta (1 ng/ml), and RANKL (150 ng/ml) for the indicated periods. Relative expression levels of RANK mRNA were determined by real time RT-PCR. Data represent mean values of three independent experiments, with error bars indicating ± SD. **P< 0.01 for adherent condition vs. non-adherent condition at the same time point. B. Left panel: BMMs grown under the adherent condition were transferred to the non-adherent condition and further cultured for the indicated time periods. Right panel: BMMs under the non-adherent condition were transferred to the adherent condition and further cultured for the indicated time periods. RANK mRNA expression was analyzed by RT-PCR. C. Left panel: comparison of RANK protein expression levels between BMMs cultured under the adherent condition and those transferred to the non-adherent condition. Right panel: Comparison of RANK protein levels between BMMs cultured under the non-adherent condition and those transferred to the adherent condition. RANK protein expression on the surface of BMMs was analyzed by flowcytometry. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23139818), licensed under a CC-BY license. Not internally tested by R&D Systems.