Mouse/Rat BMP-15/GDF-9B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5917
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse BMP-15/GDF-9B
Gln268-Arg392
Accession # Q9Z0L4
Gln268-Arg392
Accession # Q9Z0L4
Specificity
Detects mouse and rat BMP-15/GDF-9B in Western blots and recombinant mouse in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) BMP‑15 is observed and less than 1% cross‑reactivity with rhBMP-3 and rhBMP-10 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse/Rat BMP-15/GDF-9B Antibody
Detection of Rat BMP‑15/GDF‑9B by Western Blot.
Western blot shows lysates of rat ovary tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat BMP-15/GDF-9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5917) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BMP-15/GDF-9B proprecursor at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse/Rat BMP-15/GDF-9B Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Rat ovary tissue
Sample: Rat ovary tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-15/GDF-9B
BMP-15 (Bone morphogenetic protein 15; also GDF-9B) is a 24-26 kDa member of the TGF-beta superfamily of proteins. It is expressed by late primary follicle oocytes, where it promotes the transition of preantral granulosa cells to cumulus cells, and later the expansion of cumulus cells. Mouse BMP-15 proprecursor is a 50-55 kDa, 367 amino acid (aa) glycoprotein. It is proteolytically cleaved to generate a 40 kDa prosegment (aa 26-267) plus a 24 kDa, 124 aa mature region (aa 268-392) that may be phosphorylated (on Ser7 of the mature molecule) and/or glycosylated. Secreted BMP-15 does not occur as a mature homodimer, but it does exist as a mature monomer, an uncleaved proprecursor, or as a noncovalent heterodimer composed of a cleaved mature region and its prosegment. The heterodimer may also form an oligomer. The BMP-15 prosegment reportedly forms a noncovalent heterodimer with 20 kDa mature GDF-9. Mature mouse BMP-15 shares 70% and 91% aa identity with human and rat BMP-15, respectively.
Long Name
Bone Morphogenetic Protein 15
Alternate Names
BMP15, GDF-9B
Gene Symbol
BMP15
UniProt
Additional BMP-15/GDF-9B Products
Product Documents for Mouse/Rat BMP-15/GDF-9B Antibody
Product Specific Notices for Mouse/Rat BMP-15/GDF-9B Antibody
For research use only
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