Mouse/Rat Draxin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6149
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Draxin
Gly26-Ile343
Accession # Q6PAL1
Gly26-Ile343
Accession # Q6PAL1
Specificity
Detects mouse and rat Draxin in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Draxin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse/Rat Draxin Antibody
Detection of Rat Draxin by Western Blot.
Western blot shows lysates of rat embryonic hippocampal glial cells. PVDF Membrane was probed with 1 µg/mL of Mouse/Rat Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6149) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Draxin at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Draxin in Mouse Embryo.
Draxin was detected in immersion fixed frozen sections of mouse embryo (E13) using Mouse Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6149) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cells in the midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse/Rat Draxin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13)
Sample: Immersion fixed frozen sections of mouse embryo (E13)
Western Blot
1 µg/mL
Sample: Rat embryonic hippocampal glial cells
Sample: Rat embryonic hippocampal glial cells
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Draxin
Draxin (Dorsal repulsive axon guidance protein; also neucrin) is a secreted, 58 kDa, presumable glycoprotein member of the Draxin family of molecules. In mammals, it is expressed in neurons (axons), astroglia, and likely cells of the developing somite. Draxin acts as a Wnt antagonist, apparently by binding to LRP6. The net effect is to block neural crest migration, and the organization of axons into functional tracts or bundles (fasciculation). Mature mouse Draxin is 318 amino acids (aa) in length. It contains one potential N-linked glycosylation site, followed by a Cys-rich domain (aa 268-327). Mature mouse Draxin (aa 26-343) shares 80% aa identity with mature human Draxin, and 94% aa identity with mature rat Draxin.
Long Name
Dorsal Repulsive Axon Guidance Protein
Alternate Names
C1orf187, Neucrin
Gene Symbol
DRAXIN
UniProt
Additional Draxin Products
Product Documents for Mouse/Rat Draxin Antibody
Product Specific Notices for Mouse/Rat Draxin Antibody
For research use only
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