Mouse/Rat HO-1/HMOX1/HSP32 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3169
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant rat HO-1/HMOX1
Met1-Met289
Accession # P06762
Met1-Met289
Accession # P06762
Specificity
Detects mouse and rat HO-1/HMOX1/HSP32 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat HO-1/HMOX1/HSP32 Antibody
Detection of Mouse/Rat HO-1/HMOX1/HSP32 by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line, A20 mouse B cell lymphoma cell line, NRK rat normal kidney cells, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse/Rat HO‑1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3169) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Detection of Rat HO-1/HMOX1/HSP32 by Western Blot
Effect of prodigiosins on the mRNA and protein expression of HO-1 in gastric tissue.Data (means ± SEM of three assays) were normalized to the levels of GAPDH and expressed as fold induction (log2 scale), relative to the level of HO-1 in the control. aP < 0.05, significant difference vs. the health rats; bP < 0.05, significant difference vs. HCl/ethanol-induced rats, Duncan's post-hoc test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31194753), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse/Rat HO-1/HMOX1/HSP32 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: C2C12 mouse myoblast cell line, A20 mouse B cell lymphoma cell line, NRK rat normal kidney cells, and Rat-2 rat embryonic fibroblast cell line
Sample: C2C12 mouse myoblast cell line, A20 mouse B cell lymphoma cell line, NRK rat normal kidney cells, and Rat-2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4 using AF3169 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HO-1/HMOX1/HSP32
Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 (HO-1/HMOX1) and a constitutive heme oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Rat HO-1 shares 93% amino acid sequence identity with mouse HO-1.
Long Name
Heme Oxygenase 1
Alternate Names
HMOX1, HO1, HSP32
Gene Symbol
HMOX1
UniProt
Additional HO-1/HMOX1/HSP32 Products
Product Documents for Mouse/Rat HO-1/HMOX1/HSP32 Antibody
Product Specific Notices for Mouse/Rat HO-1/HMOX1/HSP32 Antibody
For research use only
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