Mouse/Rat Integrin alpha8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4076
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse, Canine, Transgenic Mouse
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha8
Phe38-Phe1007
Accession # A28ARA8
Phe38-Phe1007
Accession # A28ARA8
Specificity
Detects mouse and rat Integrin alpha8 in Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human (rh) Integrin alpha8 is observed, and less than 1% cross-reactivity with rhIntegrin alpha5, recombinant mouse (rm) Integrin alpha5, rhIntegrin alphaV and rmIntegrin alphaV is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat Integrin alpha8 Antibody
Detection of Mouse Integrin alpha8 by Western Blot.
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Integrin alpha8 in 4T1 Mouse Breast Cancer Cell Line.
Integrin a8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse and Rat Integrin alpha8 by Simple WesternTM.
Simple Western lane view shows lysates of mouse lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Applications for Mouse/Rat Integrin alpha8 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed 4T1 mouse breast cancer cell line
Sample: Immersion fixed 4T1 mouse breast cancer cell line
Simple Western
10 µg/mL
Sample: Mouse lung tissue and rat lung tissue
Sample: Mouse lung tissue and rat lung tissue
Western Blot
0.25 µg/mL
Sample: Mouse lung tissue
Sample: Mouse lung tissue
Reviewed Applications
Read 3 reviews rated 5 using AF4076 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 8
Alternate Names
ITGA8
Gene Symbol
ITGA8
UniProt
Additional Integrin alpha 8 Products
Product Documents for Mouse/Rat Integrin alpha8 Antibody
Product Specific Notices for Mouse/Rat Integrin alpha8 Antibody
For research use only
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