Mouse/Rat Jak2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2988
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Jak2
Gly2-Val63
Accession # Q62120
Gly2-Val63
Accession # Q62120
Specificity
Detects endogenous mouse and rat Jak2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat Jak2 Antibody
Detection of Mouse/Rat Jak2 by Western Blot.
Western blot shows lysates of DA3 mouse myeloma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell lines. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Jak2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2988) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Jak2 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse/Rat Jak2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: DA3 mouse myeloma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell lines
Sample: DA3 mouse myeloma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell lines
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jak2
Janus Kinase 2 (Jak2) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak2 and other family members Jak1, Jak3, and Tyk2 occurs via phosphorylation at two adjacent tyrosine residues as well as potentially one or more serine residues. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak2 plays an essential role in interferon gamma signaling.
Long Name
Janus Kinase 2
Alternate Names
EC 2.7.10, EC 2.7.10.2, JAK-2, Janus kinase 2Janus kinase 2 (a protein tyrosine kinase), JTK10, tyrosine-protein kinase JAK2
Entrez Gene IDs
3717 (Human)
Gene Symbol
JAK2
UniProt
Additional Jak2 Products
Product Documents for Mouse/Rat Jak2 Antibody
Product Specific Notices for Mouse/Rat Jak2 Antibody
This product is sold under license from Millipore Corporation under the following US or foreign patents: 5,821,069; 5,658,791; EP0560890. This product shall not be used to commercially screen drug molecules being developed as JAK1 or JAK2 inhibitors. Any such activity will be outside the scope of the research use only label license.
For research use only
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