Mouse/Rat MMP-8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3245
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat MMP-8
Leu21-Pro466
Accession # O88766
Leu21-Pro466
Accession # O88766
Specificity
Detects rat and mouse MMP-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human MMP‑8 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat MMP-8 Antibody
Detection of Rat MMP‑8 by Western Blot.
Western blot shows lysates of rat lung tissue and rat spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat MMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-8 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse/Rat MMP-8 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Rat MMP-8 (Catalog # 3245-MP), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Rat MMP-8 (Catalog # 3245-MP), see our available Western blot detection antibodies
Western Blot
0.5 µg/mL
Sample:
Sample:
Rat lung tissue and Rat spleen tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-8
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase, collagenase 2) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II, and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta-casein, and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP-8 has a high capacity for infiltrating connective tissue and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP-8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region, and a hemopexin-like domain (1).
References
- Tschesche, T. et al. (2004) Handbook of Proteolytic Enzymes (eds. A.J. Barrett et al.) p.480, Academic Press, San Diego.
Long Name
Matrix Metalloproteinase 8
Alternate Names
Collagenase 2, MMP8
Gene Symbol
MMP8
UniProt
Additional MMP-8 Products
Product Documents for Mouse/Rat MMP-8 Antibody
Product Specific Notices for Mouse/Rat MMP-8 Antibody
For research use only
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