Mouse/Rat Neurocan Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF5800

R&D Systems, part of Bio-Techne
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BAF5800
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AF5800
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG

View all Neurocan Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Neurocan
Asp23-Asp637
Accession # NP_031815

Specificity

Detects mouse and rat Neurocan in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse/Rat Neurocan Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Neurocan (Catalog # 5800-NC)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurocan

Neurocan is a 220 kDa nervous tissue-specific chondroitin sulfate proteoglycan member of the aggrecan/versican proteoglycan family (1). Mouse Neurocan is synthesized as a 1268 amino acid (aa) precursor that contains a 22 aa signal sequence and a 1246 aa mature chain. The mature chain contains one Ig-like V-type domain (aa 37-157), two Link domains (aa 159-254 and 258-356), two EGF-like domains (aa 960-996 and 998-1034), one C-type lectin-like domain (aa 1036-1165), one Sushi domain (aa 1165-1224), and five potential sites for N-linked glycosylation. Mature mouse Neurocan is 90% and 66% aa identical to mature rat and human Neurocan, respectively. Neurocan binds with high affinity to the cell adhesion molecules (CAM) Ng-CAM and N-CAM to inhibit Neuronal adhesion and neurite growth (2-3). In the developing rat retina, the expression of Neurocan is regulated both temporally and spatially, which suggests that it may play a role in the differentiation of and neural network formation of the mammalian retina (1). Injury to the CNS leads to permanent loss of function due to the inability of severed nerve fibers to regenerate back to their targets (4). The lack of CNS repair is attributed in part to the extracellular matrix chondroitin sulfate proteoglycans, such as Neurocan, which are produced by activated glial cells post-injury (4).

References

  1. Inatani, M. et al. (1999) Invest. Ophthalmol. Vis. Sci. 40:2350.
  2. Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
  3. Retzler, C. et al. (1996) J. Biol. Chem. 271:27304.
  4. Quaglia, X. et al. (2008) Brain 131:240.

Alternate Names

CSPG3, NCAN, NEUR

Entrez Gene IDs

1463 (Human); 13004 (Mouse); 58982 (Rat)

Gene Symbol

NCAN

UniProt

NP_031815

Additional Neurocan Products

Product Documents for Mouse/Rat Neurocan Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse/Rat Neurocan Biotinylated Antibody

For research use only

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