Mouse/Rat Serpin F1/PEDF Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1149
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Simple Western, Western Blot
Cited:
ELISA Development, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Serpin F1/PEDF
Asp43-Thr417
Accession # BAA31978
Asp43-Thr417
Accession # BAA31978
Specificity
Detects mouse Serpin F1/PEDF in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human Serpin F1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat Serpin F1/PEDF Antibody
Detection of Mouse and Rat Serpin F1/PEDF by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, NRK rat normal kidney cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse/Rat Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1149) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Serpin F1/PEDF at approximately 46-48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Serpin F1/PEDF by Simple WesternTM.
Simple Western lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin F1/PEDF at approximately 55 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse/Rat Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1149) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse/Rat Serpin F1/PEDF Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line
Sample: NIH‑3T3 mouse embryonic fibroblast cell line
Western Blot
0.2 µg/mL
Sample: C2C12 mouse myoblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, NRK rat normal kidney cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: C2C12 mouse myoblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, NRK rat normal kidney cell line, and Rat‑2 rat embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin F1/PEDF
Long Name
Serpine F1/Pigment Epithelium-derived Factor
Alternate Names
EPC-1, PEDF
Gene Symbol
SERPINF1
UniProt
Additional Serpin F1/PEDF Products
Product Documents for Mouse/Rat Serpin F1/PEDF Antibody
Product Specific Notices for Mouse/Rat Serpin F1/PEDF Antibody
For research use only
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