Mouse/Rat VSIG1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5324
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VSIG1
Val23-Glu234
Accession # BAE35812
Val23-Glu234
Accession # BAE35812
Specificity
Detects mouse and rat VSIG1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) VSIG1 is observed and less than 1% cross‑reactivity with recombinant mouse (rm) VSIG2, rmVSIG3, rmVSIG4, and rhVSIG3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat VSIG1 Antibody
Detection of Mouse and Rat VSIG1 by Western Blot.
Western blot shows lysates of rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat VSIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5324) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for VSIG1 at approximately 55 and 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse/Rat VSIG1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Rat testis tissue
Sample: Rat testis tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VSIG1
Long Name
V-Set and Immunoglobulin Domain Containing 1
Alternate Names
GPA34
Gene Symbol
VSIG1
UniProt
Additional VSIG1 Products
Product Documents for Mouse/Rat VSIG1 Antibody
Product Specific Notices for Mouse/Rat VSIG1 Antibody
For research use only
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