Mouse Reelin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3820
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 397919
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Reelin
Ala27-Met862
Accession # Q60841
Ala27-Met862
Accession # Q60841
Specificity
Detects mouse Reelin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Reelin Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse Reelin (Catalog # 3820-MR)
Sample: Recombinant Mouse Reelin (Catalog # 3820-MR)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Reelin
Reelin is a 400 kDa, extracellular matrix glycoprotein secreted by several neurons. It is a serine protease that degrades fibronectin and laminin. Reelin also binds lipoprotein receptor superfamily members APOER2 and VLDLR which transduce signals important for neuronal positioning during brain development and synaptic plasticity in the adult brain. In vivo, Reelin undergoes proteolytic processing at two sites, generating three Reelin fragments. The N-terminal domain binds integrin alpha3/ beta1, leading to a disruption of neuronal glial cell interactions and inhibition of neuronal migration. Mouse Reelin shares 94% and 96% amino acid identity with human and rat Reelin, respectively in the region immunized.
Alternate Names
Reeler, RELN, RL
Gene Symbol
RELN
UniProt
Additional Reelin Products
Product Documents for Mouse Reelin Antibody
Product Specific Notices for Mouse Reelin Antibody
For research use only
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